The 2-Minute Rule for thr77 login
The 2-Minute Rule for thr77 login
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is particular.
Target Information Cyclin E belongs towards the remarkably conserved cyclin family, whose members are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins function as regulators of CDK kinases. Different cyclins show distinctive expression and degradation styles which add to your temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for read more mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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